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CME Credit Offered
CME Credit Offered
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Abstract Award
Recording available 2/16-5/2
Recording available 2/16-5/2
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AIUM Credit
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Foundation Awardee
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Abstract Award
Abstract Award
Foundation Awardee
Foundation Awardee

Poster Session 4

Category: Prematurity

Poster Session 4

(1295) Amniotic IL-6 as a predictor of intra-amniotic infection: a retrospective cohort study

Thursday, February 12, 2026
3:30 PM - 5:00 PM  
Objective:
Intra-amniotic infection is the most common origin of spontaneous preterm delivery at early gestational ages. Main concern is the need to perform an amniocentesis for this indication. We aimed to compare early markers of infection in maternal blood and amniotic fluid in patients with Threatened Preterm Labor (PTL), Preterm Premature Rupture of Membranes (PPROM) and cervical incompetence.

Study Design:
A retrospective cohort study was performed in pregnant patients admitted with a diagnosis of PTL, PPROM or cervical incompetence between 2018 and 2025 who underwent amniocentesis to rule out the occurrence of intra-amniotic infection. Infection was confirmed by positive amniotic fluid cultures for aerobes, anaerobes, genital mycoplasma, or positive 16S rRNA gene sequencing. Biomarkers analyzed included maternal serum C-reactive protein (CRP) and leukocyte count, amniotic fluid Interleukin (IL)-6, and glucose concentrations. Amniotic fluid IL-6 was assessed using an automated electrochemiluminescence immunoanalyzer providing results in a few minutes. Non-parametric tests were used for group comparisons, and ROC curves assessed diagnostic performance.

Results:
The study included 74 PTL, 59 PPROM, and 29 patients with cervical incompetence. Intra-amniotic infection was present in 54 patients (25 PTL, 20 PPROM, 9 cervical incompetence). An amniotic fluid IL-6 > 13.9 ng/mL showed excellent diagnostic performance with an AUC of 0.93 (95% Confidence interval: 0.89–0.97), 88% sensitivity, and 89% specificity. Amniotic fluid glucose also showed good diagnostic p</span>erformance (AUC: 0.90, 95% CI 0.84 - 0.96), while accuracy of maternal CRP (AUC: 0.79 95% CI 0.71 - 0.86) and leukocytes (AUC: 0.56, 95% CI 0.46- 0.66) were limited.

Conclusion:
Amniotic fluid IL-6 showed the best diagnostic performance to predict intra-amniotic infection in patients with PTL, PPROM, and cervical incompetence, outperforming traditional inflammatory markers in maternal blood. This rapid point-of-care might help in early diagnosis and clinical decision-making in these high-risk pregnancies.