(491) Development of Therapeutics for Preterm Premature Rupture of Membranes Using Regenerative Medicine

Preterm premature rupture of membranes (PPROM) is a major cause of spontaneous preterm birth and associated neonatal complications, including cerebral palsy. Currently, no effective treatment exists to restore the integrity of ruptured fetal membranes. This study aimed to develop a novel biomaterial-based therapy using shape-adaptive, tissue-adhesive hydrogels to physically seal and regenerate ruptured membranes, enabling prolonged pregnancy.

Study Design:

We developed a chitosan-based hydrogel film enriched with plant-derived polyphenols to form a biocompatible, adhesive patch. The material was engineered to seal ruptured amniotic membranes and support tissue integration. In vitro experiments assessed patch retention and cellular infiltration. In vivo, a PPROM model was established by rupturing the amniotic membrane in pregnant mice at embryonic day 15. The hydrogel patch was applied to the rupture site, and therapeutic efficacy was evaluated based on membrane healing and pregnancy duration.

Results:

The hydrogel patch formed a stable adhesive film at room temperature, maintaining attachment for at least seven days and degrading gradually. It adhered securely to the membrane across defects of varying sizes. Histological analysis confirmed cellular infiltration and tissue compatibility. In vivo, patch application restored membrane continuity and significantly prolonged pregnancy to term compared with controls.

Conclusion:

This study demonstrates the therapeutic potential of a bioadhesive hydrogel patch for treating PPROM. The patch promotes membrane regeneration and prevents preterm birth in a murine model. Future work should explore integrating drug delivery systems to enhance antimicrobial and regenerative effects for clinical application.